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Novice Karate Group (ages 8 & up)

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Ruben Lavrentiev
Ruben Lavrentiev

Naked Women Mature

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Can immature oocytes found in the medium after cortical tissue preparation for fertility preservation, constitute a reliablesource of mature eggs? GV oocytes released during cortical tissue preparation and mature to MII stage with an average23% success rate, resulting in average 8 MII oocytes per patient. Cryopreservation of ovarian tissue by slow freezing has becomea reliable method of fertility preservation for many groups of the patients. During the procedure of cortical tissue preparation,many small antral follicles within the tissue are cut open resulting in release of immature oocytes into the medium. Theseoocytes have the potential to develop into mature oocytes that may be used for fertility purposes. The aim of present study wasto improve the IVM protocol for oocytes deriving from small follicles which was previously rarely used in the context of fertilityutilization. Oocyte-Cumulus Complexes (COCs) were collected into HEPES-buffered medium supplemented with 10 mg/mLof human serum albumin and cultured following an IVM protocol established in our laboratory (315 oocytes). After 48 hoursoocytes were denudated and assessed for maturation stage 22. A total of 9 patients aged 21-36 years (mean age 29) who hadone ovary excised for fertility preservation were included. After collection GV oocytes were divided into one of three groups:COCs with large amount of cumulus cells, small amount of cumulus cells and naked oocytes. After maturation measurementsof oocyte diameter, diameter of egg??s cytoplasm and zona pellucida thickness were taken and related to the maturation status ofthe oocyte. On average, 35 immature oocytes per patient from one ovary were collected with the range of 13-60 oocytes. 23%(N=74) of all oocytes matured to the MII stage within 48 hours. On average each patient had 8.2 mature oocytes. Maturationrate for the oocytes from large COCs was 37.5%, small COCs matured with the rate of 22.7%, while success rate for the nakedones was only 6.3% (p 041b061a72


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